Glucosylated nucleotide sequences from T2-bacteriophage deoxyribonucleic acid

Glucosylated Nucleotide Sequences from T-even Bacteriophage Deoxyribonucleic Acids.

1. The frequencies of various pyrimidine nucleotide sequences in phage-T2VG111 DNA and phage-T6 DNA have been measured. The hydroxymethylcytosine nucleotides that bear glucosyl groups in phage-T2VG111 DNA and gentiobiosyl groups in phage-T6 DNA are not randomly distributed. 2. Sequences in which two or three hydroxymethylcytosine nucleotides are terminated at both ends by purine nucleotides bea...

Deoxyribonucleic acid repair in bacteriophage.

Separation of T-even bacteriophage deoxyribonucleic acid from host deoxyribonucleic acid by hydroxyapatite chromatography.

A simple and rapid method is described for separation of T-even bacteriophage deoxyribonucleic acid (DNA) from host (Escherichia coli) DNA by hydroxyapatite column chromatography with a shallow gradient of phosphate buffer at neutral pH. By this method, bacteriophage T2, T4, and T6 DNA (but not T5, T7, or lambda DNA) could be separated from host E. coli DNA. It was found that glucosylation of t...

Correlation between degradation of bacteriophage T2 deoxyribonucleic acid and the resistance of Escherichia coli to infection.

The ability of certain strains of Escherichia coli to degrade T2 deoxyribonucleic acid to acid-soluble fragments is correlated with their high capacity to survive T2 infection.

Complete nucleotide sequence of the cohesive ends of bacteriophage P2 deoxyribonucleic acid.

At the ends of bacteriophage P2 DNA, the S’-terminated strands are 19 nucleotides longer than the 3’-terminated strands. The complete nucleotide sequence of the two cohesive ends of P2 DNA has been determined. This was accomplished by repair synthesis, using Escherichia coIi DNA polymerase and labeled deoxyribonucleoside triphosphates with or without a ribonucleoside triphosphate, followed by p...